Abstract: The influence regularities of Mo, Cr, Zr and Si elements on the transformation point, microstructure and property of new α+β titanium alloy were studied by optical microscope（OM）, scanning electron microscope（SEM）, universal testing machine and fatigue testing machine. The results show that Cr element has more significant role in stablilizing β phase than Mo element. Cr element can significantly increase the transformation point. The as-cast microstructure of five titanium alloys is all the lamellar microstructure. 0.1wt% Si element is beneficial to refine grain and shorten columnar zone. 3wt% Cr and 3wt% Mo elements are beneficial to refine lamellar microstructure. 3wt% Cr,1wt% Zr,0.1wt% Si and 3wt% Mo elements are beneficial to precipitate the fine strip α phase. A new TC32 titanium alloy with well matched properties of strength,plasticity and fatigue is obtained under the strengthening effect of Mo, Cr, Zr and Si elements.