Abstract: Electron beam welding tests of laser solid formed GH4169 superalloy with anisotropic microstructure were carried out. The effects of the crystal orientation difference of base metals on the microstructure and tensile deformation behavior of the welded joint were analyzed. The results show that the dendrite grows along the direction perpendicular to the fusion line in the welded sample with 0° differential orientation, while the electron beam welding metal with 45°differential orientation is asymmetrically distributed due to the influence of orientation and heat dissipation direction of the base metals at both sides. The orientation of both sides of the electron beam welding metal with 90° differential orientation is different, and the grain size is different greatly. The tensile test results show that the increase of orientation difference leads to the decrease of weld strength and plasticity. The KAM analysis show that, compared with other welds, the weld with 90° orientation difference has the largest amount of non-deformable structures, and the plasticity is poor. Moreover,the Schmidt factor at the center of the weld is larger, which is the easiest to start slip, so the strength is low.