Abstract: For 2 MW direct-drive wind turbine, based on the blade element mormentum, the change rule of wind turbine tower load and tower bolt stress under the action of turbulent wind was explored at different wind speeds law, the blade imbalance load on the wind turbine tower bolt load-stress response was expored, and based on the wind turbine one year of SCADA data, the blade imbalance degree of the distribution of the influence of the law of the fatigue damage distribution of the tower bolts was analysed. The results show that, under the relative φ_b=0° blade equilibrium state, among the 100 high-strength bolts on the tower cross-section 10 m above the bottom of the tower of the wind turbine, the fatigue damage of the high-strength bolts in the E position is largest, with the 20-year fatigue cumulative damage value of 0.577; when the blade imbalance degree φ_b=-4° and φ_b=4°, the fatigue cumulative damage value of the high-strength bolts in the E position is respectively 0.649 and 0.676, and the fatigue damage ratio of E-position high-strength bolts under blade imbalance increased by 12.48% and 17.16%, respectively, indicating that the blade imbalance load increases the fatigue damage of wind turbine tower bolts and reduces the safety of wind turbines.